mtc multiple tissue cdna panel  (TaKaRa)

Journal: Journal of Biomedical Science

Article Title: Involvement of an alternatively spliced mitochondrial oxodicarboxylate carrier in adipogenesis in 3T3-L1 cells

doi: 10.1186/1423-0127-16-92

Figure Lengend Snippet: RT-PCR analysis of ODC-AS expression in 3T3-L1 cells . Expression of ODC-AS and ODC mRNA in 3T3-L1 Cells were detected with RT-PCR in mRNAs prepared at the indicated days from 3T3-L1 cell pre- and after differentiation, and visualized under UV after agarose gel electrophoresis with ethidium bromide. 1 kb DNA standards is shown on the right. The addition of differentiation reagents was indicated at the bottom. G3PDH was amplified as an internal control and shown below. L: 1 kb DNA ladder; PC: positive control for ODC from cloned cDNA plasmid; NC: negative control for G3PDH (water).

Article Snippet: Expression of ODC and ODC-AS was analyzed by PCR amplification of mouse Multiple Tissue cDNA panel (MTC, Clontech, Palo Alto, CA) with specific primers (5'ODC: 5'-CCCTGGTCGCGAAGATGTC-3', 5'ODC-AS: 5'-CAGTCGACCTACGTACCATG-3' and common 3' primer: 5'-CGATTGACTTGCAAGCCAAC-3') at the following conditions: 40 cycles of 95°C for 30 seconds, 68°C for 90 seconds with extension at 72°C for 3 minutes using Advantage II PCR kit (Clontech) (Additional file ).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis, Amplification, Positive Control, Clone Assay, Plasmid Preparation, Negative Control